Investigation of the genetic structure of some common bean (Phaseolus vulgaris L.) commercial varieties and genotypes used as a genitor with SSR and SNP markers
نویسندگان
چکیده
Common bean is a species belonging to the Phaseolus genus of Leguminosae family. It has economic importance due being rich in protein, vitamin A and C, minerals. Being one most cultivated legumes, determination genetic diversity genotypes or populations an important role terms our resources. The objective this study was evaluate structure 94 which were different parts world country with SSR SNP markers. 10 loci 73 primers used for commercial cultivars breeding lines. All found be polymorphic. total 89 alleles identified loci. Mean number per locus (Na = 8.9), effective allele (Ne 3.731), Shannon information index (I 1.468), observed heterozygosity (Ho 0.023), expected (He 0.654) calculated based on analysis. According results Bayesian-based STRUCTURE analysis using data, genetically divided into three main clusters. distance UPGMA dendrogram obtained from analysis, 2 clusters corresponding Mesoamerican Andean gene pools. provide about structures studied With results, it will possible develop programs new by
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Integration of simple sequence repeat (SSR) markers into a molecular linkage map of common bean (Phaseolus vulgaris L.).
Microsatellite or simple sequence repeat (SSR) markers have been successfully used for genomic mapping, DNA fingerprinting, and marker-assisted selection in many plant species. Here we report the first successful assignment of 15 SSR markers to the Phaseolus vulgaris molecular linkage map. A total of 37 SSR primer pairs were developed and tested for amplification and product-length polymorphism...
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Glutamine synthetase (GS) is an octameric enzyme. The nodule cytosol of the common bean (Phaseolus vulgaris L.) has two major types of GS subunit polypeptides ([beta] and [gamma]). As a result, nine different isozymes containing varied proportions of [beta] and [gamma] can be generated. The isozymes are resolvable by native polyacrylamide gel electrophoresis. Staining the gel for GS activity re...
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The common bean is the most important grain legume for direct human consumption in the world and BCMV is one of the world's most serious bean diseases that can reduce yield and quality of harvested product. To determine the best tolerance index to BCMV and recognize tolerant genotypes, 2 experiments were conducted in field conditions. Twenty five common bean genotypes were sown in 2 separate RC...
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ژورنال
عنوان ژورنال: Genetic Resources and Crop Evolution
سال: 2022
ISSN: ['0925-9864', '1573-5109']
DOI: https://doi.org/10.1007/s10722-022-01396-5